The boiling point of some liquids can also change to quite close to room temperature, increasing the evaporation loss dramatically. The air pressuredecreases at higher altitudes and the conversion factor Z decreases as well. The geographic altitude affects the accuracy through the air pressure. Typically, the density of water is 0.996 kg/dm3, for ethanol 0.79 kg/dm3 and for sulfuric acid (H2SO4) 1.85 kg/dm3. The density varies according to thetemperature and air pressure. If the temperature of the liquid, pipette and air is the same, the accuracy is not significantly affected.ĭensity is the mass/volume ratio of the liquid. The figure below shows the change in volume when the liquid has a different temperature than the pipette and air. The most important factor in pipetting accuracy is the liquid temperature. To empty the tip completely the operating button is pressed to the second stop (blow out).įactors affecting the accuracy of Air Displacement Pipettes: When the operating button is pressed to the first stop again, the air dispensesthe liquid.
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